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FEBS Lett. 2007 Nov 27;581(28):5413-7. Epub 2007 Oct 29.

Function of a separate NH3-pore in Aquaporin TIP2;2 from wheat.

Author information

1
Institute of Botany, Applied Plant Science, Darmstadt University of Technology, Schnittspahnstrasse 10, D-64287 Darmstadt, Germany.

Abstract

Functional analysis of heterologously expressed TaTIP2;2 by means of stopped-flow spectrometric studies provide evidence for water and ammonia conductivity. A series of experiments under increasing pH indicate that the gaseous NH(3), rather than the ammonium ion NH(4)(+) was transported. Results from inhibitor studies strongly suggest that NH(3) is not transported in file with water, but through a separate pathway, which could be supplied by the 5th central pore in a tetramer conformation.

PMID:
17967420
DOI:
10.1016/j.febslet.2007.10.034
[Indexed for MEDLINE]
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