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Nat Methods. 2007 Nov;4(11):943-50. Epub 2007 Oct 28.

Two-photon photostimulation and imaging of neural circuits.

Author information

1
Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, Box 2435, New York, New York 10027, USA. vn59@columbia.edu

Abstract

We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity.

PMID:
17965719
DOI:
10.1038/nmeth1105
[Indexed for MEDLINE]

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