Abstract
The yeast retrovirus-like element Ty3 GAG3 gene encodes a Gag3 polyprotein analogous to retroviral Gag. Gag3 lacks matrix, but contains capsid, spacer, and nucleocapsid domains. Expression of a Ty3 Gag3 or capsid domain optimized for expression in Escherichia coli was sufficient for Ty3 particle assembly. Virus-like ordered particles assembled from Gag3 were similar in size to immature particles from yeast and contained nucleic acid. However, particles assembled from the CA domain were variable in size and displayed much less organization than native particles. These results indicate that assembly can be driven through interactions among capsid subunits in the particle, but that the nucleocapsid domain, likely in association with RNA, confers order upon this process.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adaptor Proteins, Signal Transducing
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Base Sequence
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Capsid Proteins / chemistry
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Capsid Proteins / genetics
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Capsid Proteins / physiology
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / physiology
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Cloning, Molecular
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DNA Primers / genetics
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Escherichia coli / genetics
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Gene Expression
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Genes, Fungal
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Microscopy, Atomic Force
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RNA-Directed DNA Polymerase / chemistry
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RNA-Directed DNA Polymerase / genetics*
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RNA-Directed DNA Polymerase / physiology
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Retroelements / genetics
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Retroelements / physiology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / physiology
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Saccharomyces cerevisiae / virology
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / genetics*
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Saccharomyces cerevisiae Proteins / physiology
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Transformation, Genetic
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Virion / chemistry
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Virion / genetics
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Virion / physiology
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Virus Assembly
Substances
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Adaptor Proteins, Signal Transducing
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Capsid Proteins
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Carrier Proteins
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DNA Primers
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MDV1 protein, S cerevisiae
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Recombinant Proteins
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Retroelements
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Saccharomyces cerevisiae Proteins
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RNA-Directed DNA Polymerase
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reverse transcriptase Ty3, S cerevisiae