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Methods Enzymol. 2007;433:51-72.

Noninvasive assessment of the role of cyclooxygenases in cardiovascular health: a detailed HPLC/MS/MS method.

Author information

1
Institute for Translational Medicine and Therapeutics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Abstract

A robust method for the routine quantitation of a selected group of urinary eicosanoid metabolites of interest to cardiovascular research in human and mouse is described and discussed. Included are the addition of stable isotope-labeled internal standards, solid phase extraction, and quantitation by liquid chromatography/tandem mass spectrometry using selected reaction monitoring (SRM) techniques.

PMID:
17954228
DOI:
10.1016/S0076-6879(07)33003-6
[Indexed for MEDLINE]

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