Format

Send to

Choose Destination
Proteomics. 2007 Nov;7(22):4066-8.

Constitution and quantity of lysis buffer alters outcome of reverse phase protein microarrays.

Author information

1
Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.

Abstract

Application of novel technology to clinical samples requires optimization of procedures. Reverse phase protein lysate arrays use femtomolar quantities of tissue lysate from clinical samples with which to profile biochemical events happening in the tumor. We analyzed the effects of different tissue solubilization buffers on frozen ovarian tumor samples in order to identify the system with the best signal intensity dynamic range, reproducibility, tissue solubility, and signal consistency. A modified RIPA-like buffer supplemented with DTT and SDS was deemed optimal.

PMID:
17952872
DOI:
10.1002/pmic.200700484
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center