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Nat Methods. 2007 Nov;4(11):927-9. Epub 2007 Oct 21.

A monoclonal antibody for G protein-coupled receptor crystallography.

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1
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 279 Campus Drive, Stanford, Palo Alto, California 94305, USA.

Abstract

G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.

PMID:
17952087
DOI:
10.1038/nmeth1112
[Indexed for MEDLINE]
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