Format

Send to

Choose Destination
Methods Mol Biol. 2007;400:571-85.

Ion-channel reconstitution.

Author information

1
Centro de Estudios Cientificos, Valdivia, Chile.

Abstract

In this chapter, a detailed protocol is given for ion-channel reconstitution in the two most used model membranes: planar bilayers and liposomes. In the planar bilayer section, methods are described for the expression of ion channels in Xenopus laevis oocytes, the isolation of their membranes, the insertion of ion channels into the bilayer by vesicle fusion, and the recording of single-ion channel current measurements at a constant applied voltage. The reconstitution of bacterial channels in liposomes is also given. It includes the expression and purification of bacterial channels in E. Coli host strain XL1-blue, the insertion of the channels in liposomes, and the recording of their currents by patch clamping.

PMID:
17951760
DOI:
10.1007/978-1-59745-519-0_38
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center