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Diagn Microbiol Infect Dis. 2008 Feb;60(2):217-9. Epub 2007 Oct 22.

Rapid identification and specific quantification of Staphylococcus epidermidis by 5' nuclease real-time polymerase chain reaction with a minor groove binder probe.

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1
Department of Bacteriology, The Jikei University School of Medicine, Tokyo 105-8461, Japan. iwase.tadayuki@jikei.ac.jp

Abstract

A species-specific quantitative detection method involving 5' nuclease real-time polymerase chain reaction using a minor groove binder probe that was designed from the sodA gene was developed for Staphylococcus epidermidis. This method distinguished S. epidermidis from other staphylococci and specifically quantified the bacterium. This study shows that the method is useful for the identification and quantitative detection of S. epidermidis.

[Indexed for MEDLINE]

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