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Genetics. 2007 Dec;177(4):2519-23. Epub 2007 Oct 18.

The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe.

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1
Section of Eukaryotic Transposable Elements, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Abstract

Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.

PMID:
17947404
PMCID:
PMC2219505
DOI:
10.1534/genetics.107.081075
[Indexed for MEDLINE]
Free PMC Article
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