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J Clin Microbiol. 2007 Dec;45(12):4018-20. Epub 2007 Oct 17.

Agar dilution method for detection of inducible clindamycin resistance in Staphylococcus spp.

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1
Department of Microbiology, Royal North Shore Hospital, Sydney, New South Wales, Australia.

Abstract

We describe the development and validation of an agar dilution method for the detection of inducible clindamycin resistance by using 227 previously characterized erythromycin-resistant, clindamycin-susceptible Staphylococcus sp. isolates. Mueller-Hinton agar with defibrinated horse blood containing a range of erythromycin concentrations (1 to 8 mg/liter) combined with clindamycin at 0.5 mg/liter was used to determine the optimal concentration that produced growth of inducible isolates while inhibiting that of isolates without the inducible phenotype. A concentration of clindamycin of 0.5 mg/liter with erythromycin at 1 mg/liter was the optimal combination for detection of inducible resistance and resulted in a sensitivity of 100% (95% confidence interval [CI], 97.9 to 100) and a specificity of 100% (95% CI, 93.0 to 100). Attention must be paid to ensuring that a sufficient inoculum has been used, since an inoculum below the standard 10(7) bacteria/ml may result in false-negative results. This method has been incorporated into routine use in our laboratory.

PMID:
17942656
PMCID:
PMC2168581
DOI:
10.1128/JCM.01158-07
[Indexed for MEDLINE]
Free PMC Article
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