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Biochem Biophys Res Commun. 2007 Dec 7;364(1):181-6. Epub 2007 Oct 1.

Cucurbitacin glucosides: antioxidant and free-radical scavenging activities.

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The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.


The cucurbitacins are of great interest because of the wide range of biological activities they exhibit in plants and animals. We studied the antioxidant properties of cucurbitacin B + E glucosides (cucurbitacin glucoside combination, CGC) and their direct free-radical scavenging properties, using ESR spectroscopy. Antioxidant activity was measured by the ability of the CGC to reduce preformed ABTS*+ into its native form and to inhibit MDA formation during the oxidation of linoleic acid. In both methods, the CGC exhibited antioxidant activity in a dose-dependent manner as expected from antioxidants. Using ESR spectroscopy, we found that the CGC inhibited *OH-dependent DEPMPO-OH adduct formation, O2*--dependent DEPMPO-OOH adduct formation, and the 1O2-dependent TEMPO adduct generated in the photoradiation-porphin system. The IC50 values were 0.38, 8, and 11 mM, respectively. Together, these data demonstrate that the CGC exhibits antioxidant properties, probably through the involvement of a direct scavenging effect on several free radicals.

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