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Environ Health Perspect. 2007 Oct;115(10):1467-73.

A phytoestrogen-rich diet increases energy expenditure and decreases adiposity in mice.

Author information

1
Department of Genetic Medicine and Development and National Center for Competence in Research - Frontiers in Genetics, Faculty of Medicine, University of Geneva, Geneva, Switzerland.

Abstract

BACKGROUND:

Obesity is an increasingly prevalent health problem, and natural effective therapeutic approaches are required to prevent its occurrence. Phytoestrogens are plant-derived compounds with estrogenic activities; they can bind to both estrogen receptors alpha and beta and mimic the action of estrogens on target organs.

OBJECTIVES:

The purpose of this study was to examine the influence of soy-derived phytoestrogens on energy balance and metabolism.

METHODS:

Male outbred mice (CD-1) were allowed ad libitum access to either a high soy-containing diet or a soy-free diet from conception to adulthood. We measured circulating serum isoflavone levels using reverse-phase solid-phase extraction for subsequent liquid chromatography electrospray tandem mass spectrometry analysis. Adult animals were analyzed for body composition by dual-energy X-ray absorptiometry, locomotor activity by running-wheel experiments, respiratory exchange rate by indirect calorimetry, and food intake using metabolic cages. Quantitative reverse transcriptase-polymerase chain reaction was performed to determine the expression of hypothalamic neuropeptide genes.

RESULTS:

We found that adult mice fed a soy-rich diet had reduced body weight, adiposity, and resistance to cold. This lean phenotype was associated with an increase in lipid oxidation due to a preferential use of lipids as fuel source and an increase in locomotor activity. The modulation of energy balance was associated with a central effect of phytoestrogens on the expression of hypothalamic neuropeptides, including agouti-related protein.

CONCLUSION:

The data suggest that dietary soy could have beneficial effects on obesity, but they also emphasize the importance of monitoring the phytoestrogen content of diets as a parameter of variability in animal experiments.

KEYWORDS:

AgRP; endocrine disruptors; isoflavones; lipid oxidation; obesity; phytoestrogens

PMID:
17938737
PMCID:
PMC2022650
DOI:
10.1289/ehp.10413
[Indexed for MEDLINE]
Free PMC Article

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