Stage 12c flowers were emasculated 24 h before ovules were stained for GUS expression. Ovules from plants transformed with promoterless GUS expression vector had no detectable GUS expression (A). Approximately 50% of ovules in T1 plants expressing GUS from one of four DUF784 promoters (B–E) or from one of four DUF1278 promoters (F–I) had a single locus of GUS expression near the micropyle, consistent with expression in the synergids, and in some cases possibly also the egg cell. Expression of GUS from a DEFL promoter (J) or one of two PSIL promoters (K, L) resulted in GUS expression within the central cell. (B) At5g35405::GUS, (C) At4g08025::GUS, (D) At5g34885::GUS, (E) At2g21727::GUS, (F) At5g54062::GUS, (G) At2g14378::GUS, (H) At5g36340::GUS, (I) At5g42895::GUS, (J) At4g14276::GUS, (K) At4g24974::GUS, and (L) At5g27238::GUS.