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Mol Cell. 2007 Oct 12;28(1):159-66.

Do DEAD-box proteins promote group II intron splicing without unwinding RNA?

Author information

1
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.

Abstract

The DEAD-box protein Mss116p promotes group II intron splicing in vivo and in vitro. Here we explore two hypotheses for how Mss116p promotes group II intron splicing: by using its RNA unwinding activity to act as an RNA chaperone or by stabilizing RNA folding intermediates. We show that an Mss116p mutant in helicase motif III (SAT/AAA), which was reported to stimulate splicing without unwinding RNA, retains ATP-dependent unwinding activity and promotes unfolding of a structured RNA. Its unwinding activity increases sharply with decreasing duplex length and correlates with group II intron splicing activity in quantitative assays. Additionally, we show that Mss116p can promote ATP-independent RNA unwinding, presumably via single-strand capture, also potentially contributing to DEAD-box protein RNA chaperone activity. Our findings favor the hypothesis that DEAD-box proteins function in group II intron splicing as in other processes by using their unwinding activity to act as RNA chaperones.

PMID:
17936712
PMCID:
PMC2062517
DOI:
10.1016/j.molcel.2007.07.028
[Indexed for MEDLINE]
Free PMC Article

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