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J Microbiol Methods. 2007 Dec;71(3):336-9. Epub 2007 Sep 17.

Ethidium monoazide and propidium monoazide for elimination of unspecific DNA background in quantitative universal real-time PCR.

Author information

1
Institute of Milk Hygiene, Milk Technology and Food Science, Department of Veterinary Public Health and Food Science, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria. ingeborg.hein@vu-wien.ac.at

Abstract

Unspecific background DNA in quantitative universal real-time PCR utilizing a hydrolysis probe was completely suppressed by the addition of EMA or PMA to the PCR mix via cross-linking of the dyes to DNA during 650 W visible light exposure. The proposed procedure had no effect on the sensitivity of the real-time PCR reaction.

PMID:
17936386
DOI:
10.1016/j.mimet.2007.09.005
[Indexed for MEDLINE]

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