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Arch Oral Biol. 2008 Feb;53(2):150-4. Epub 2007 Oct 23.

Sec translocase and sortase A are colocalised in a locus in the cytoplasmic membrane of Streptococcus mutans.

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1
The Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, China. gloriaping@126.com

Abstract

INTRODUCTION:

The general secretory (Sec) pathway is the main pathway for protein transport. Sortase A has been shown to anchor surface protein containing an LPXTG motif to the cell wall. In order to know the distribution pattern of the Sec translocons and sortase A in Streptococcus mutans, we explored the subcellular localisation of SecA and sortase A.

METHODS:

The anti-SecA antibody and anti-sortase A antibody were examined on Western blots to evaluate specificities. An indirect post-embedding immunogold method was used to determine the subcellular localisation of the Sec pathway and sortase A enzyme in S. mutans membrane.

RESULTS:

Immunoblotting experiments showed that the anti-SecA antibody and anti-sortase A antibody specifically recognised a single band of about 95 kDa and 27 kDa, respectively in the S. mutans GS-5 cell lysates. Immunogold electron micrographs showed that small and large gold particles clustered together at a distinct microdomain.

CONCLUSIONS:

These data showed for the first time that Sec translocase and sortase A colocalised to a single locus in the cytoplasmic membrane of S. mutans GS-5.

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