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Curr Opin Chem Biol. 2007 Oct;11(5):543-52.

Self-sacrifice in radical S-adenosylmethionine proteins.

Author information

1
Department of Chemistry, The Pennsylvania State University, University Park, PA 16803, United States. sjb14@psu.edu

Abstract

The radical SAM superfamily of metalloproteins catalyze the reductive cleavage of S-adenosyl-l-methionine to generate a 5'-deoxyadenosyl radical (5'-dA*) intermediate that is obligate for turnover. The 5'-dA* acts as a potent oxidant, initiating turnover by abstracting a hydrogen atom from an appropriate substrate. A special class of these enzymes use this strategy to functionalize unactivated C-H bonds by insertion of sulfur atoms. This review will describe the characterization of three members of this class - biotin synthase, lipoyl synthase, and MiaB protein - each of which has been shown to cannibalize itself during turnover.

PMID:
17936058
PMCID:
PMC2637762
DOI:
10.1016/j.cbpa.2007.08.028
[Indexed for MEDLINE]
Free PMC Article
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