Send to

Choose Destination
Small. 2007 Nov;3(11):1950-6.

Using the ring-shaped protein TRAP to capture and confine gold nanodots on a surface.

Author information

Protein Design Laboratory, Yokohama City University, Tsurumi, Suehiro 1-7-29, Yokohama 230-0045, Japan.


The cavity of the toroidal protein TRAP (trp RNA-binding attenuation protein) is modified to capture gold nanodots in solution. By engineering a titanium-binding peptide onto one surface of the ring it is also possible to bind it specifically and tightly to a TiO2 surface. TRAP bound in this way is then used to capture gold nanodots and attach them to prepared surfaces. Gold-protein complexes are observed using atomic force microscopy and transmission electron microscopy. The modified TRAP is used to build gold nanodots into the SiO2 layer of a metal oxide semiconductor. This is the first use of a ring protein, rather than the more commonly used spherical protein cages, to constrain nanodots to a surface. This method is an important addition to the current range of bionanotechnology tools and may be the basis for future, multicomponent electronic devices.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center