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J Microbiol Methods. 2007 Dec;71(3):332-5. Epub 2007 Sep 21.

New transposon delivery plasmids for insertional mutagenesis in Bacillus anthracis.

Author information

1
The Scripps Research Institute, Department of Molecular and Experimental, Medicine, Division of Cellular Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States.

Abstract

Two new transposon delivery vector systems utilizing Mariner and mini-Tn10 transposons have been developed for in vivo insertional mutagenesis in Bacillus anthracis and other compatible Gram-positive species. The utility of both systems was directly demonstrated through the mutagenesis of a widely used B. anthracis strain.

PMID:
17931726
PMCID:
PMC2175031
DOI:
10.1016/j.mimet.2007.09.006
[Indexed for MEDLINE]
Free PMC Article

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