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J Cereb Blood Flow Metab. 2008 Apr;28(4):747-51. Epub 2007 Oct 10.

GADD34 gene restores virulence in viral vector used in experimental stroke study.

Author information

1
Division of Clinical Neuroscience, Wellcome Surgical Institute, Garscube Estate, University of Glasgow, Glasgow, UK. cmc39v@clinmed.gla.ac.uk

Abstract

GADD34 is expressed in the ischaemic brain and reverses protein synthesis shutdown. Consequently, GADD34 could have neuroprotective potential in stroke. BHK medium, a replication-deficient HSV viral vector (HSV1716) with no insert or containing full-length GADD34, the N terminal or a conserved portion of the gene, was injected into mouse brain before stroke. Infarct size was 1.0+/-0.26, 1.19+/-0.36, 1.5+/-0.36, 1.3+/-0.36, and 1.1+/-0.28 mm3, respectively. The increase in infarct size with full-length GADD34 was statistically significant (P<0.05). Immunohistochemistry confirmed viral protein expression. Tissue culture studies revealed GADD34 gene restored virulence in HSV1716, suggesting that HSV virulence, rather than increased GADD34, exacerbated ischaemic damage.

PMID:
17928799
DOI:
10.1038/sj.jcbfm.9600565
[Indexed for MEDLINE]

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