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Differentiation. 2008 Mar;76(3):245-52. Epub 2007 Oct 9.

Involvement of phosphatidylserine externalization in the down-regulation of c-myb expression in differentiating C2C12 cells.

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Institute of Molecular Genetics, AS CR v.v.i., Vídenská 1083, Prague 4, CZ-14220, Czech Republic.


Analysis of c-myb gene down-regulation in differentiating C212 cells revealed that in proliferating cells, c-myb expression is high and ceases as the proliferation rate decreases. However, a low level of c-myb mRNA was detected in confluent non-proliferating differentiating cells for an extended period of time before it declined to an undetectable level. The time course of c-myb gene silencing in differentiating cells correlated with exposition of phosphatidylserine (PS) on the cell surface. Moreover, the interaction of exposed PS with exogenously added annexin V perturbed PS-mediated cell signaling and transiently up-regulated the declining c-myb expression. We, therefore, suggest that cell surface-exposed PS, which plays a role in the process of myotube formation, is also involved in the down-regulation of c-myb expression.

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