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Appl Microbiol Biotechnol. 2007 Dec;77(3):513-23. Epub 2007 Oct 9.

Yeast expression platforms.

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  • 1Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, 06466, Gatersleben, Germany.


Yeasts provide attractive expression platforms. They combine ease of genetic manipulations and the option for a simple fermentation design of a microbial organism with the capabilities of an eukaryotic organism to secrete and to modify a protein according to a general eukaryotic scheme. For platform applications, a range of yeast species has been developed during the last decades. We present in the following review a selection of established and newly defined expression systems. The review is concluded by the description of a wide-range vector system that allows the assessment of the selected organisms in parallel for criteria like secretion or appropriate processing and modification in a given case.

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