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Cancer Lett. 2007 Dec 8;258(1):144-53. Epub 2007 Oct 17.

Proanthocyanidin from grape seeds inactivates the PI3-kinase/PKB pathway and induces apoptosis in a colon cancer cell line.

Author information

1
Department of Physiological Sciences, Stellenbosch University, Stellenbosch, South Africa. ame@sun.ac.za

Abstract

The aim of this investigation was to evaluate the chemopreventative/antiproliferative potential of a grape seed proanthocyanidin extract (GSPE) against colon cancer cells (CaCo2 cells) and to investigate its mechanism of action. GSPE (10-100 microg/ml) significantly inhibited cell viability and increased apoptosis in CaCo2 cells, but did not alter viability in the normal colon cell line (NCM460). The increased apoptosis observed in GSPE-treated CaCo2 cells correlated with an attenuation of PI3-kinase (p110 and p85 subunits) and decreased PKB Ser(473) phosphorylation. GSPE might thus exert its beneficial effects by means of increased apoptosis and suppression of the important PI3-kinase survival-related pathway.

PMID:
17923279
DOI:
10.1016/j.canlet.2007.08.020
[Indexed for MEDLINE]

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