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Neuron. 2007 Oct 4;56(1):43-57.

Imaging large-scale neural activity with cellular resolution in awake, mobile mice.

Author information

1
Department of Molecular Biology, Carl Icahn Labs, Princeton University, Princeton, NJ 08544, USA.

Abstract

We report a technique for two-photon fluorescence imaging with cellular resolution in awake, behaving mice with minimal motion artifact. The apparatus combines an upright, table-mounted two-photon microscope with a spherical treadmill consisting of a large, air-supported Styrofoam ball. Mice, with implanted cranial windows, are head restrained under the objective while their limbs rest on the ball's upper surface. Following adaptation to head restraint, mice maneuver on the spherical treadmill as their heads remain motionless. Image sequences demonstrate that running-associated brain motion is limited to approximately 2-5 microm. In addition, motion is predominantly in the focal plane, with little out-of-plane motion, making the application of a custom-designed Hidden-Markov-Model-based motion correction algorithm useful for postprocessing. Behaviorally correlated calcium transients from large neuronal and astrocytic populations were routinely measured, with an estimated motion-induced false positive error rate of <5%.

PMID:
17920014
PMCID:
PMC2268027
DOI:
10.1016/j.neuron.2007.08.003
[Indexed for MEDLINE]
Free PMC Article

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