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Mol Cell Probes. 1991 Aug;5(4):281-4.

Specific detection of the toxic shock syndrome toxin-1 gene using the polymerase chain reaction.

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Institut de Bactériologie, Faculté de Médecine, France.


A rapid and specific assay for toxic shock syndrome toxin-1 gene (tst gene) detection in Staphylococcus aureus was developed using the polymerase chain reaction. A two-primer set and an oligonucleotide detection probe were synthesized. After 40 cycles of amplification, detection of a 160-bp amplified DNA fragment was carried out by agarose gel electrophoresis and Southern blot hybridization. This assay was sensitive since it was able to detect 1-10 bacteria. It was also specific since no amplification was documented with DNAs from enterotoxigenic S. aureus or Gram-negative bacteria devoid of the tst gene.

[Indexed for MEDLINE]

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