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Mol Microbiol. 1991 Oct;5(10):2529-39.

Physical and genetic map of the Neisseria gonorrhoeae strain MS11-N198 chromosome.

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Max-Planck-Institut für Biologie, Abteilung Infektionsbiologie, Tübingen, Germany.


A macro-restriction map of the Neisseria gonorrhoeae chromosome was constructed using the enzymes Nhel and Spel. Combinations of one- and two-dimensional electrophoresis of completely or partially digested chromosomal DNA were performed to align the restriction fragments. The chromosome is circular, with an estimated size of 2.33 Mb +/- 35 kb. A genetic map was derived from the physical map; positions of over 60 defined loci were determined by Southern hybridization.

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