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J Struct Biol. 2007 Nov;160(2):249-62. Epub 2007 Aug 29.

Fast, robust, and accurate determination of transmission electron microscopy contrast transfer function.

Author information

1
Unidad de Biocomputación, Centro Nacional de Biotecnología (CSIC), Campus Universidad Autónoma s/n, 28049 Cantoblanco, Madrid, Spain. coss.eps@ceu.es

Erratum in

  • J Struct Biol. 2012 Nov;180(2):387.

Abstract

Transmission electron microscopy, as most imaging devices, introduces optical aberrations that in the case of thin specimens are usually modeled in Fourier space by the so-called contrast transfer function (CTF). Accurate determination of the CTF is crucial for its posterior correction. Furthermore, the CTF estimation must be fast and robust if high-throughput three-dimensional electron microscopy (3DEM) studies are to be carried out. In this paper we present a robust algorithm that fits a theoretical CTF model to the power spectrum density (PSD) measured on a specific micrograph or micrograph area. Our algorithm is capable of estimating the envelope of the CTF which is absolutely needed for the correction of the CTF amplitude changes.

PMID:
17911028
DOI:
10.1016/j.jsb.2007.08.013
[Indexed for MEDLINE]

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