Polymerization of the actin-like protein MamK, which is associated with magnetosomes

Azuma Taoka; Ryuji Asada; Long-Fei Wu; Yoshihiro Fukumori

doi:10.1128/JB.00899-07

Polymerization of the actin-like protein MamK, which is associated with magnetosomes

J Bacteriol. 2007 Dec;189(23):8737-40. doi: 10.1128/JB.00899-07. Epub 2007 Sep 28.

Authors

Azuma Taoka¹, Ryuji Asada, Long-Fei Wu, Yoshihiro Fukumori

Affiliation

¹ Department of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.

Abstract

The recombinant actin-like protein MamK was purified from Escherichia coli and used as an antigen to generate the anti-MamK antibody. Immunostaining studies showed a linear distribution of MamK in Magnetospirillum magnetotacticum cells and of MamK in association with magnetosomes. Moreover, we demonstrated that MamK polymerizes into filamentous bundles in vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / chemistry
Bacterial Proteins / chemistry*
Bacterial Proteins / genetics
Bacterial Proteins / immunology
Bacterial Proteins / metabolism*
Biopolymers / chemistry
Biopolymers / metabolism
Cytoskeleton / metabolism*
Magnetospirillum / cytology*
Magnetospirillum / metabolism*
Organelles / metabolism*
Protein Transport

Substances

Actins
Bacterial Proteins
Biopolymers