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Spine J. 2007 Sep-Oct;7(5):601-8. Epub 2007 Jan 2.

Glucosamine HCl alters production of inflammatory mediators by rat intervertebral disc cells in vitro.

Author information

1
Department of Orthopaedic Surgery, Orthopaedic Bioengineering Laboratory, University of California, San Francisco, 533 Parnassus Ave., Box 0514, San Francisco, CA 94143-0514, USA.

Abstract

BACKGROUND CONTEXT:

Studies on cartilage have shown anti-inflammatory effects of glucosamine related to inhibition of inflammatory mediators. Intradiscal injection of glucosamine has been proposed as a treatment for chronic discogenic low back pain. However, there have been no studies of the direct effects of glucosamine on disc cells.

PURPOSE:

To determine the effects of glucosamine HCl on pro-inflammatory mediator production by intervertebral disc cells.

STUDY DESIGN:

An in vitro, experimental study of interleukin-1 (IL-1) stimulated rat intervertebral disc cells treated with and without glucosamine HCl.

METHODS:

Rat annulus and nucleus cells were cultured in alginate beads and exposed to IL-1a (10 ng/mL)+glucosamine HCl (4.5 mg/mL), IL-1 alone, or neither for 4 and 7 days. Cell viability and IL-6, tumor necrosis factor alpha (TNF-alpha), prostaglandin E(2) (PGE(2)), and NO levels in the medium were quantified and compared across treatments.

RESULTS:

Annulus cells, 7 days: Glucosamine completely inhibited IL-6 and TNF-alpha, increased NO (by 75%), and reduced viability (by 89%) compared with IL-1 alone. Nucleus cells, 7 days: Glucosamine reduced IL-6 (by 89%), PGE(2) (91%), and NO (90%) with no effect to viability.

CONCLUSIONS:

Glucosamine inhibits inflammatory mediator production by IL-1 stimulated disc cells, but also adversely affects the viability of rat annulus cells. The response is cell-type dependent, illustrated by differences for annulus and nucleus cells.

PMID:
17905323
DOI:
10.1016/j.spinee.2006.10.012
[Indexed for MEDLINE]

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