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Genome Biol. 2007;8(9):R203.

A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.

Author information

1
New York University School of Medicine/Cancer Institute, Department of Pharmacology, First Avenue, New York, NY 10016, USA. ramanuj.dasgupta@med.nyu.edu

Abstract

Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes.

PMID:
17903264
PMCID:
PMC2375041
DOI:
10.1186/gb-2007-8-9-r203
[Indexed for MEDLINE]
Free PMC Article

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