Two distinct human POM121 genes: requirement for the formation of nuclear pore complexes

FEBS Lett. 2007 Oct 16;581(25):4910-6. doi: 10.1016/j.febslet.2007.09.021. Epub 2007 Sep 21.

Abstract

Pom121 is one of the integral membrane components of the nuclear pore complex (NPC) in vertebrate cells. Unlike rodent cells carrying a single POM121 gene, human cells possess multiple POM121 gene loci on chromosome 7q11.23, as a consequence of complex segmental-duplications in this region during human evolution. In HeLa cells, two "full-length" Pom121 are transcribed and translated by two distinct genetic loci. RNAi experiments showed that efficient depletion of both Pom121 proteins significantly reduces assembled NPCs on nuclear envelope. Pom121-depletion also induced clustering of NPCs, indicating its role on maintenance of NPC structure/organization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Flanking Region
  • Base Sequence
  • Cell Nucleus / ultrastructure
  • Chromosome Mapping
  • Cloning, Molecular
  • Exons
  • Gene Duplication*
  • HeLa Cells
  • Humans
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / metabolism
  • Molecular Sequence Data
  • Nuclear Pore / ultrastructure*
  • Protein Structure, Tertiary
  • RNA Interference

Substances

  • Membrane Glycoproteins
  • POM121 protein, human