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Nat Chem Biol. 2007 Nov;3(11):707-8. Epub 2007 Sep 23.

Sortagging: a versatile method for protein labeling.

Author information

1
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.

Abstract

Genetically encoded reporter constructs that yield fluorescently labeled fusion proteins are a powerful tool for observing cell biological phenomena, but they have limitations. Sortagging (sortase-mediated transpeptidation) is a versatile chemoenzymatic system for site-specific labeling of proteins with small (<2 kDa) probes. Sortagging combines the precision of a genetically encoded tag with the specificity of an enzymatic reaction and the ease and chemical versatility of peptide synthesis. Here we apply this technique to proteins in vitro and on the surface of living cells.

PMID:
17891153
DOI:
10.1038/nchembio.2007.31
[Indexed for MEDLINE]

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