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Spectrochim Acta A Mol Biomol Spectrosc. 2007 Dec 15;68(4):1029-35. Epub 2007 Aug 11.

UV Raman spectroscopy--a technique for biological and mineralogical in situ planetary studies.

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Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, D-07743 Jena, Germany.


We report on the great advantages of using deep UV Raman system for in situ planetary applications. Among them are to be mentioned: (I) higher scattering efficiency compared to VIS-IR Raman excitation wavelengths, (II) electronic resonance effects which increase the intrinsically weak Raman signal thus improving the S/N ratio of the detected Raman signals and (III) spectral separation of Raman and fluorescence signals. All these advantages are making UV Raman a valuable technique for in situ planetary applications. Mineral as well as biological samples were analyzed using Raman deep UV excitation and the results are presented. For the mineral samples a comparison with excitation in the NIR-VIS spectral regions is made. The impact of fluorescence on Raman data acquisition at different laser excitation wavelengths is assessed. Making use of the resonance effects, spectra of microorganisms were recorded with a high S/N ratio, allowing afterwards a very precise identification and classification (to the strain level) of the measured samples.

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