Send to

Choose Destination
See comment in PubMed Commons below
Cell Cycle. 2007 Sep 15;6(18):2276-83. Epub 2007 Jul 9.

A transgenic mouse model for high content, cell cycle phenotype screening in live primary cells.

Author information

Department of Gynecology and Obstetrics, Stanford University School of Medicine, Stanford, California 94305, USA.


High content cell-based genetic and small molecule library screens are powerful strategies in drug discovery and investigations of disease mechanisms. We report that primary cells derived from a transgenic mouse model expressing a fluorescence mitosis biosensor provide unambiguous phenotype readouts without the need for transfection or immunocytochemistry. Phenotype profiles of cell cycle disruption and of apoptosis are easily detectable at a single time point selected from time-lapse live fluorescence microscopy. Most importantly, this transgenic mouse model may be crossed with cancer mouse models to derive biosensor-expressing primary cancer cells for use in high content screening strategies targeting discovery of tumor-specific chemotherapeutic compounds.

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Taylor & Francis
    Loading ...
    Support Center