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Cell Cycle. 2007 Sep 15;6(18):2276-83. Epub 2007 Jul 9.

A transgenic mouse model for high content, cell cycle phenotype screening in live primary cells.

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1
Department of Gynecology and Obstetrics, Stanford University School of Medicine, Stanford, California 94305, USA.

Abstract

High content cell-based genetic and small molecule library screens are powerful strategies in drug discovery and investigations of disease mechanisms. We report that primary cells derived from a transgenic mouse model expressing a fluorescence mitosis biosensor provide unambiguous phenotype readouts without the need for transfection or immunocytochemistry. Phenotype profiles of cell cycle disruption and of apoptosis are easily detectable at a single time point selected from time-lapse live fluorescence microscopy. Most importantly, this transgenic mouse model may be crossed with cancer mouse models to derive biosensor-expressing primary cancer cells for use in high content screening strategies targeting discovery of tumor-specific chemotherapeutic compounds.

PMID:
17881898
DOI:
10.4161/cc.6.18.4718
[Indexed for MEDLINE]
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