PQQ protects against H2O2 in rat cardiac myocytes. Acutely isolated adult rat cardiac myocytes grown on laminin-coated glass coverslips were exposed to control (vehicle-treated), H2O2, or H2O2 + PQQ. Control – vehicle treatment; H2O2 (1 mM) for 10 min; PQQ (10 μM) pretreatment for at least 30 minutes prior to experiment.
(A) Acutely isolated rat cardiac myocytes treated with from vehicle (control), H2O2 (1 mM and 100 μM), ± bovine serum albumin (BSA).
(B) Rat cardiac myocytes incubated with 0.1% BSA were assayed for cell viability, and mean values (± SEM) from control, H2O2, and H2O2+PQQ experimental groups are represented. Approximately 400-600 cells were counted in a total of 10 fields per dish for each experimental group. Three rat hearts were used for each of the conditions shown in the bargraph.
(C) Representative images of acutely isolated rat cardiac myocytes from control, H2O2, and H2O2+PQQ experimental groups. Live cells were rod-shaped and excluded the vital dye trypan blue, indicating an intact plasma membrane. Dead cells were contracted and stained positive for trypan blue, indicating a compromised plasma membrane. ** p<0.01 compared to control.