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Antimicrob Agents Chemother. 2007 Dec;51(12):4512-4. Epub 2007 Sep 17.

Biochemical Characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase.

Author information

1
Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.

Abstract

The carbapenem-hydrolyzing beta-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.

PMID:
17875998
PMCID:
PMC2168006
DOI:
10.1128/AAC.00491-07
[Indexed for MEDLINE]
Free PMC Article

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