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Opt Lett. 2007 Sep 15;32(18):2726-8.

Two-photon fluorescence bioimaging with an all-semiconductor laser picosecond pulse source.

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  • 1SONY Corporation, Atsugi, Japan.


We have demonstrated successful two-photon excitation fluorescence bioimaging using a high-power pulsed all-semiconductor laser. Toward this purpose, we developed a pulsed light source consisting of a mode-locked laser diode and a two-stage diode laser amplifier. This pulsed light source provided optical pulses of 5 ps duration and having a maximum peak power of over 100 W at a wavelength of 800 nm and a repetition frequency of 500 MHz.

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