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Science. 2007 Sep 14;317(5844):1563-6.

Quantitative imaging of nitrogen fixation by individual bacteria within animal cells.

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1
National Resource for Imaging Mass Spectrometry, Harvard Medical School, and Brigham and Women's Hospital, 65 Landsdowne Street, Cambridge, MA 02139, USA. cpl@harvard.edu

Erratum in

  • Science. 2007 Oct 26;318(5850):570.

Abstract

Biological nitrogen fixation, the conversion of atmospheric nitrogen to ammonia for biosynthesis, is exclusively performed by a few bacteria and archaea. Despite the essential importance of biological nitrogen fixation, it has been impossible to quantify the incorporation of nitrogen by individual bacteria or to map the fate of fixed nitrogen in host cells. In this study, with multi-isotope imaging mass spectrometry we directly imaged and measured nitrogen fixation by individual bacteria within eukaryotic host cells and demonstrated that fixed nitrogen is used for host metabolism. This approach introduces a powerful way to study microbes and global nutrient cycles.

PMID:
17872448
DOI:
10.1126/science.1145557
[Indexed for MEDLINE]
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