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J Antimicrob Chemother. 2007 Nov;60(5):973-80. Epub 2007 Sep 13.

Demonstration of in vivo transfer of doxycycline resistance mediated by a novel transposon.

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1
King's College London Dental Institute at Guy's, King's College and St Thomas' Hospitals, Infection Research Group, Guy's Campus, London SE1 9RT, UK.

Abstract

OBJECTIVES:

The aim of this study was to investigate the transfer of bacterial doxycycline resistance between oral bacteria in subjects receiving systemic doxycycline for the treatment of periodontitis.

PATIENTS AND METHODS:

Streptococci were cultured before and after treatment from the subgingival plaque of two patients with periodontitis, genotyped and investigated for the presence of antimicrobial resistance determinants and conjugative transposons.

RESULTS:

In one subject, a strain of Streptococcus sanguinis resistant to doxycycline was a minor component of the pre-treatment streptococcal flora but dominated post-treatment. In a second subject, a strain of Streptococcus cristatus, which was sensitive to doxycycline before treatment, was found to have acquired a novel conjugative transposon during treatment, rendering it resistant to doxycycline and erythromycin. The novel transposon, named CTn6002, was sequenced and found to be a complex element derived in part from Tn916, and an unknown element which included the erythromycin resistance gene erm(B). A strain of Streptococcus oralis isolated from this subject pre-treatment was found to harbour CTn6002 and was therefore implicated as the donor.

CONCLUSIONS:

This is the first direct demonstration of transfer of antimicrobial resistance carried on a conjugative transposon between oral bacteria during systemic antimicrobial treatment of periodontitis in humans.

PMID:
17855723
DOI:
10.1093/jac/dkm331
[Indexed for MEDLINE]
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