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Acta Obstet Gynecol Scand. 2007 Oct;86(10):1263-8.

Sulforaphane induces growth arrest and apoptosis in human ovarian cancer cells.

Author information

1
Division of Gynecologic Oncology, Department of Obstetrics, Gynecology and Reproductive Medicine, Mount Sinai School of Medicine, 1176 Fifth Avenue, Box 1173, New York, NY 10029, USA. linus.chuang@mssm.edu

Abstract

OBJECTIVES:

Isothiocyanates (ITC) from broccoli and other cruciferous vegetables have long been shown to have chemopreventive properties, as demonstrated in cancer models in rodents. Sulforaphane (SFN) is a major ITC present in broccoli. We examined the effects of SFN on the growth of the OVCAR-3 and SKOV-3 ovarian carcinoma cell lines.

METHODS:

Cell cycle phase determination was performed using a Coulter flow cytometer. DNA strand breaks in apoptotic cells were measured by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end-labelling (TUNEL).

RESULTS:

There was a concentration dependent decrease in cell density. Approximately 50% decrease was observed after 48 h of incubation with SFN (2 μM). Analysis of cell cycle phase progression revealed a decrease in the cell populations in S and G2M phases, with an increase of G1 cell population, indicating a G1 cell cycle arrest. The degree of decrease in the replicating population was concentration and time dependent. Incubation of OVCAR-3 cells in cultures with concentrations of 2, 10 and 50 μM of SFN showed 6, 8 and 17% apoptosis, respectively. In addition, when OVCAR-3 cells were exposed to SFN for various time periods (1, 2 or 3 days), the percentage of cells undergoing apoptosis was directly proportional to the incubation period. In this regard, while 18% of the cells underwent apoptosis after 2 days, 42% of the cells showed apoptosis after 3 days of incubation.

CONCLUSIONS:

These results clearly demonstrated an effect of SFN in inducing growth arrest and apoptosis in ovarian carcinoma cell lines.

PMID:
17851821
DOI:
10.1080/00016340701552459
[Indexed for MEDLINE]
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