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Anal Bioanal Chem. 2007 Nov;389(5):1633-8. Epub 2007 Sep 12.

Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Author information

1
Analytical Chemistry Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA. johanna.camara@nist.gov

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized to discriminate between wild-type and ampicillin-resistant Escherichia coli. Only ampicillin-resistant E. coli displayed an m/z approximately 29,000 peak, which was confirmed as beta-lactamase by in-gel digestion followed by peptide mass fingerprinting. Rapid MALDI-TOF MS detection of antibiotic-resistance could fulfill an important clinical need, providing critical phenotypic information beyond genus-species identification.

PMID:
17849103
DOI:
10.1007/s00216-007-1558-7
[Indexed for MEDLINE]

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