Format

Send to

Choose Destination
See comment in PubMed Commons below
Biometals. 2008 Jun;21(3):259-71. Epub 2007 Sep 11.

Siderotyping of fluorescent Pseudomonas: molecular mass determination by mass spectrometry as a powerful pyoverdine siderotyping method.

Author information

1
Département Génétique Moléculaire, Génomique, Microbiologie, UMR 7156, CNRS/Université Louis-Pasteur, 28 rue Goethe, 67000 Strasbourg, France. meyer@gem.u-strasbg.fr

Abstract

The numerous pyoverdines so far characterized as siderophores of fluorescent Pseudomonas could be usually differentiated one from each others by the two physico-chemical and physiological methods of siderotyping, i.e., siderophore-isoelectrofocusing and siderophore-mediated iron uptake. As shown in the present paper, the structural diversity of the peptide chain characterizing these molecules results in a very large panel of molecular masses representing 64 different values ranging from 889 to 1,764 Da for the 68 compounds included in the study, with only a few structurally different compounds presenting an identical molecular mass. Thus, the molecular mass determination of pyoverdines through mass spectrometry could be used as a powerful siderotyping method.

PMID:
17846862
DOI:
10.1007/s10534-007-9115-6
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center