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Science. 1982 Oct 15;218(4569):229-35.

Microholography of living organisms.


By using intense pulsed coherent x-ray sources that are currently under development, it will be possible to obtain magnified three-dimensional images of elementary biological structures in the living state at precisely defined instants. For optimum contrast, sensitivity, and resolution, the hologram should be made with x-rays tuned to a resonance of nitrogen near 0.3 nanometer. Resolution will then be limited mainly by the hydrodynamic expansion that occurs while the necessary number of photons is being registered. Problems of technique are also briefly discussed.

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