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Am J Obstet Gynecol. 2007 Nov;197(5):516.e1-6. Epub 2007 Sep 10.

Hypoxia regulates the expression of fatty acid-binding proteins in primary term human trophoblasts.

Author information

1
Department of Obstetrics and Gynecology, Washington University in St Louis School of Medicine, St Louis, MO, USA.

Abstract

OBJECTIVE:

Fatty acids (FAs) are essential for fetal development. Cellular FA uptake is modulated by fatty acid-binding proteins (FABPs). We hypothesized that hypoxia regulates the expression of FABPs in human trophoblasts.

STUDY DESIGN:

Primary term human trophoblasts were cultured for 72 hours in either standard (O2 = 20%) or hypoxic (O2 < 1%) conditions. FABP expression was interrogated using polymerase chain reaction and Western immunoblotting. Trophoblast lipid droplets were examined using dipyrromethene boron difluoride 493/503 staining.

RESULTS:

We detected the expression of FABP1, -3, -4, -5, and pm but not FABP2 or FABP6-9 subtypes in trophoblasts. Exposure to hypoxia markedly increased lipid droplet accumulation in trophoblasts. Consistent with this observation, hypoxia enhanced the expression of FABP1, -3, and -4. Lastly, agonists of peroxisome proliferator-activated receptor-gamma enhanced the expression of FABP1 and -4 in trophoblasts.

CONCLUSION:

Hypoxia enhances the expression of FABP1, -3, and -4 in term human trophoblasts, suggesting that FABPs support fat accumulation in the hypoxic placenta.

PMID:
17826730
PMCID:
PMC2151846
DOI:
10.1016/j.ajog.2007.03.066
[Indexed for MEDLINE]
Free PMC Article

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