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Appl Microbiol Biotechnol. 2007 Nov;77(2):339-46. Epub 2007 Sep 6.

Hyaluronic acid production by recombinant Lactococcus lactis.

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  • 1Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan.

Abstract

Microbial hyaluronic acid (HA), commonly produced by pathogenic Streptococcus, was made possible to be produced by a generally recognized as safe Lactococcus lactis by coexpressing HA synthase and uridine diphosphate-glucose dehydrogenase (UDP-GlcDH) of Streptococcus equi subsp. zooepidemicus in a nisin-controlled expression (NICE) system. With scarce expressed HA synthase alone, the constructed recombinant L. lactis (LL-NA) strain could produce HA with a concentration about 0.08 g/l in the M17 medium supplemented with 1% (w/v) glucose. In contrast to HA synthase, UDP-GlcDH of Streptococcus could be overexpressed in the NICE system. With coexpression of heterologous UDP-GlcDH with HA synthase, the constructed LL-NAB strain grew slightly slower to a concentration about 10% lower that of the LL-NA strain. However, the HA concentration produced was enhanced about eightfold to 0.65 g/l.

PMID:
17805528
DOI:
10.1007/s00253-007-1153-z
[PubMed - indexed for MEDLINE]
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