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Biosens Bioelectron. 2007 Dec 15;23(5):627-32. Epub 2007 Aug 3.

Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics.

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Institute for Lasers, Photonics and Biophotonics, Departments of Electrical Engineering, State University of New York at Buffalo, Buffalo, NY 14260, USA.


In this study, a novel phase-sensitive surface plasmon resonance (SPR) setup, based on temporal modulation of a pumping beam by a photoelastic modulator, and subsequent extraction of phase information at the second and the third harmonics of the modulation frequency, has been developed to study biomolecular interactions on SPR-supporting gold. We demonstrated that the design setup provides ultra-high phase sensitivity, together with a wide dynamic range of measurements. In particular, the proposed scheme was used to study real-time interaction of biotin-protein and streptavidin-BSA complexes. We have found that the proposed technique has a detection limit as high as 2.89 x 10(-7) in terms of refractive index units (RIU). In terms of biosensing performance, a detection sensitivity of 1.3 nM from the streptavidin-maleimide/thiolated BSA complex binding reaction has also been demonstrated.

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