Exo1 deletion prolongs the lifespan and improves maintenance of intestinal epithelia in aging telomere dysfunctional mice. A) Survival curves for mTerc^+/+, Exo1^+/+ (n=89), mTerc^+/+, Exo1^−/− (n=27), G3, Exo1^+/+ (n=25), and G3, Exo1^−/− mice (n=34). Note that G3, Exo1^−/− mice show a significantly prolonged lifespan compared to G3, Exo1^+/+ (p<0.0001). B) Tumor free survival curves for the indicated mouse cohorts. There was a mild increase in tumor formation in G3mTerc^−/−, Exo1^+/+ mice compared to mTerc^+/+, Exo1^+/+ mice (p=0.03). Exo1 deletion did not accelerate tumor formation in G3mTerc^−/−, Exo1^−/− mice. C) Representative photographs of whole mount staining of the colon of 12–15 month old mice of the indicated genotypes (magnification bar: 500μm). D) Histogram on the number of crypts per low power vision field (35x) in the colon of 12–15 and 24 month old mice of the indicated genotypes. The number of crypts was counted on whole mount staining (n=4−6 mice per group). E) Representative photographs of H&E stained longitudinal sections of the small intestine of 12–15 month old mice of the indicated genotypes (magnification bar: 200μm). F) Histogram on the number of basal crypts per vision field (100x) in the small intestine of 12–15 and 24 month old mice of the indicated genotypes (n=4−5 mice per group). Note that Exo1 deletion rescues the depletion of colon crypts and basal crypts in small intestine of 12–15 month old G3 mTerc mice, but these phenotypes appear in 20–24 month old G3mTerc^−/−, Exo1^−/− mice.

A) Representative photographs of H&E stained longitudinal sections of the spleen of 12–15 month old mice of the indicated genotypes (magnification bar: 500μm). The lymphocyte containing white pulp is circled. B) Histogram on the frequency of B-lymphocytes in spleen of 12–15 and 24 month old mice of the indicated genotypes (n=4−6 mice/group). C) Histogram showing the total number of B-lymphocytes (B220⁺) and T-lymphocytes (CD8⁺ or CD4⁺) in total bone marrow (collected from 2 hind legs) of 12–15 month and 24 month old mice of the indicated genotypes (n=4–8 mice/group). D) FACS plot showing B-lymphocytes (B220+) in bone marrow of 12–15 month old mice of the indicated genotypes. E) Histogram showing the total number of thymocytes of 12–15 month old mice of the indicated genotypes (n=5). Note that the loss of thymocytes in G3, Exo1^+/+ is rescued in G3, Exo1^−/− mice. F) Representative FACS plots showing T- cell development in thymus of 12–15 month old mice of the indicated genotypes. Note that the percentage of CD8⁺, CD4⁺ double positive T-cells (immature T-cells) is reduced in G3, Exo1^+/+ but rescued in G3, Exo1^−/− mice.

A) Representative photographs of BrdU-stained crypts in the small intestine of 12–15 month old mice of the indicated genotypes. B) Histogram showing the percentage of BrdU and PCNA-negative crypts in the small intestine of 12–15 and 24 month old mice of the indicated genotypes (n=4–5 mice/group). C) Representative photographs of apoptotic cells (green nuclei) in basal crypts (circled) of the small intestine of 12–15 month old mice of the indicated genotypes. D) Histogram showing the number of TUNEL-positive nuclei per basal crypts in the small intestine of 12–15 and 24 month old mice of the indicated genotypes (n=4–5 mice/group).

A) Telomere length was analyzed by qFISH in basal crypts of the small intestine of 12–15 month old mice (n=4/group) of the indicated genotypes. The histograms show the distribution of telomere fluorescence intensities (TFI) over all analyzed nuclei ofmTerc^+/+, Exo1^+/+ (n= 276 nuclei), mTerc^+/+, Exo1^−/− (n=160 nuclei), G3, Exo1^+/+ (n=242 nuclei) and G3, Exo1^−/− mice (n=263 nuclei). The red dotted lines indicate the mean value of each genotype. The black line marks the threshold of critically short telomeres (TFI <200 arbitrary units). B) Histogram on the rate of telomere-free ends per metaphase in bone marrow cells of 12–15 month old mice of the indicated genotypes (n=4–5). C) Representative photographs of a metaphase of a G3, Exo1^−/− mouse, white arrows pointing on telomere-free ends (magnification bar: 4 pm). D) Histogram on the rate of anaphase bridges per total number of anaphases in basal crypts of small intestine of 12–15 month old mice of the indicated genotypes. E) Representative photograph showing an anaphase bridge in basal crypts of small intestine. F) Representative photograph showing SKY-analysis of metaphase spreads from bone marrow of 24 month old G3mTerc^−/−, Exo1^−/− mice. No aberrations were detected in 40 metaphases from G3, Exo1^−/−mice (n=4).

A) Representative photographs showing γ-H2AX positive cells (white arrows) in basal crypts of 12–15 month old mice of the indicated genotypes. Magnification bars 50μm. The inlet shows a nucleus containing γ-H2AX foci at high power magnification. B) Histogram showing the percentage of γ-H2AX positive basal crypts in 12–15 and 24 month old mice of the indicated genotypes (n= 4–5 mice/group). C) Representative photographs showing 53BP1 foci (white arrows) in basal crypts of 12–15 month old mice of the indicated genotypes. Magnification bars 50μm. The inlet shows a nucleus containing 53BP1 foci at high power magnification. D) Histogram showing the number of 53BP1 foci per basal crypts in 12–15 and 24 month old mice of the indicated genotypes (n= 4–5 mice/group). E) Representative photographs showing p53 positive cells (white arrows) in basal crypts of 12–15 month old mice of the indicated genotypes. Magnification bars 200 pm. F) Histogram showing the percentage of p53 positive basal crypts in 12–15 and 24 month old mice of the indicated genotypes (n= 4–5 mice/group). G) Representative photographs showing p21-positive nuclei (red arrows) in basal crypts of 12–15 month old mice of the indicated genotypes. Magnification bars 200 μm. H) Histogram showing the percentage of p21-positive intestinal basal crypts of 12–15 and 24 month old mice of the indicated genotypes (n=4–5 mice/group).

A) Representative photographs of BrdU-positive cells or TUNNEL positive cells in the intestinal basal crypts of 4 month old mice of the indicated genotypes: 24 hours after 4Gy γ-irradiation (IR). Magnification bars: 200 μm. B,C) Histogram showing the number of BrdU-positive cells per crypt (B) or TUNNEL positive cells per crypt (C) in the small intestine of 4 month old irradiated (IR) mice of the indicated genotypes (n=5 mice/group). D) Histogram showing the relative reduction of BrdU positive cells in 15Gy irradiated (24 hours after IR) compared to non- irradiated cells of the indicated genotypes. E) Histogram showing the relative increase in cells in G1 stage of cell cycle in 15Gy irradiated (24 hours after IR) compared to non- irradiated cells of the indicated genotypes. F) Survival curve of mouse embryonic stem cells of the indicated genotypes at 3–4 days after 6TG treatment at indicated doses. Survival curves for Exo1^−/− (complete gene knockout) and Exo1^{exonΔ−/−} (knockout of the nuclease domain, which was used throughout this study) were generated in two separate experiments. The survival curves for Msh2^−/− and WT are the averaged survival curves from two separate experiments. G) Histogram showing the number γH2AX foci per nuclei in the small intestine of 4 month old mice (n=5 per group) of the indicated genotypes: 24 hours after 4Gy γ-irradiation (IR). H) Histogram showing the number of 53BP1 foci per crypt in the small intestine of 4 month old mice (n=5 per group) of the indicated genotypes: 24 hours after 4Gy γ-irradiation (IR). I) Western blots showing expression of phosphorylated ATR (Ser345), phosphorylated Chk2 (T68), and phosphorylated p53 (Ser15) along with β-actin as a loading control in mouse ear fibroblasts of the indicated genotypes at 24hrs after 15Gy irradiation and in non-irradiated controls.

A) Representative photograph showing co-localization of ssDNA (stained by non-denaturing BrdU staining) and laser induced DNA breaks (stained by a phospho-BRCA1-antibody) in mTerc^+/+, Exo1^+/+ MEFs, but absence of such co-localization in mTerc^+/+, Exo1^−/− MEFs. B) Histogram showing the percentage of laser-induced DNA breaks (phospho-BRCA1) staining positive for ssDNA (non-denaturing BrdU) in MEFs of the indicated genotypes. C) Representative photograph showing γH2AX-formation and recruitment of RPA to laser induced DNA breaks in mTerc^+/+, Exo1^+/+ MEFs. Note that γH2AX-formation is independent of the Exo1-genotype but recruitment of RPA is reduced in mTerc^+/+, Exo1^−/− MEFs. D) Histogram showing the percentage of laser-induced DNA breaks staining positive for RPA in MEFs of the indicated genotypes. E) Representative photographs showing ATR-foci in nuclei of cells in basal crypts of 12–15 month old mice of the indicated genotypes (magnification bar 50 μm). F) Histogram showing the percentage of ATR positive basal crypts in 12–15 and 24 month old mice of the indicated genotypes (n= 4–5 mice/group).