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New Microbiol. 2007 Jul;30(3):308-12.

Development and validation of a molecular method for the diagnosis of medically important fungal infections.

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1
Laboratorio di Microbiologia e Virologia, Diagnostica e Ricerca San Raffaele, Milano. demarco.donata@hsr.it

Abstract

The increasing incidence of severe fungal infections highlights the need for rapid and precise identification methods in clinical mycology. The aim of this study was to develop and validate a culture-indipendent molecular approach that could allow the detection of fungal pathogens in clinical samples, with particular attention to the identification of drug-resistant Candida and Aspergillus species. A real-time multiplex PCR assay was developed using TaqMan probes specific for highly discriminating ITS sequences. In its multiplex format the assay showed a high specificity, clearly discriminating among different species, as well as a high sensitivity (20 CFU/1 mL sample), making it a potentially useful starting point for the development of a more complete molecular diagnostic assay.

PMID:
17802916
[Indexed for MEDLINE]
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