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Autophagy. 2008 Jan;4(1):61-2. Epub 2007 Aug 7.

Chromosomal mapping of the GFP-LC3 transgene in GFP-LC3 mice.

Author information

1
Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo, Japan; SORST, Japan Science and Technology Agency, Kawaguchi, Japan.

Abstract

GFP-LC3 mice are widely used to monitor autophagy. Here, we report chromosomal mapping of the GFP.LC3 transgene in this mouse model. The transgene was located in a very distal region of chromosome 2, immediately upstream of a putative open reading frame that is thought to be a part of a pseudogene. With this information, we were able to generate a PCR system to easily determine zygosity. Homozygous GFP-LC3 mice (GFP-LC3(tg/tg)) were healthy, fertile and did not show an abnormal phenotype. Thus, GFP-LC3 integration in this model appears not to affect functions of other genes, justifying use of this mouse model.

PMID:
17786029
[Indexed for MEDLINE]

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