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Zhonghua Yu Fang Yi Xue Za Zhi. 2007 Jun;41 Suppl:43-6.

[Methylation specific binding activity of zinc finger protein Kaiso].

[Article in Chinese]

Author information

1
Peking University School of Oncology and Beijing Institute for Cancer Research, Beijing 100036, China.

Abstract

OBJECTIVE:

To compare binding activity of different zinc finger domain of human Kaiso with methylated CpG.

METHODS:

pGEX constructs with different human Kaiso domain were generated and then corresponding fusion proteins were induced and purified. Electrophoretic mobility shift assays were applied to evaluate the binding activity of fusion proteins with methylated CpG.

RESULTS:

The purified GST-KaisoZF fusion protein (without the POZ protein binding domain) could bind with methylated CpG probe specifically, but not for three or two zinc fingers without flanking domains.

CONCLUSION:

Human zinc finger protein Kaiso could bind with methylated CpG specifically, only in the assistance of the neighboring flank sequence of the zinc finger domain.

PMID:
17767857
[Indexed for MEDLINE]

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